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While PacBio SMRT sequencing technology is primarily used for methylation analysis of microbial genomes ( 3 Early screening and detection of methylation markers in cancer 3.1 Digestive system cancer 3.1.1 Colorectal cancer (CRC) According to the 2020 Global Cancer Data Statistics, colorectal cancer ranks third among common cancers worldwide and second among cancer-related mortality worldwide (Sung et al., 2021)

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