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Some of these errors waste a few percent of your peptide

To verify localization of CIPp-18 in cell membranes, cells were incubated with CIPp-18 bound to unlabeled albumin for 15 min at 37 C followed by an additional 15 min incubation in serum-free media containing 5 M Prodan, a cell membrane probe [37]

Within the brain, DSIP is particularly abundant in the hypothalamus, hippocampus, and limbic regions, where it interacts with multiple peptide and non-peptide mediators, including corticotropin-like intermediate peptide (CLIP), adrenocorticotrophic hormone (ACTH), melanocyte-stimulating hormone (MSH), and melanin-concentrating hormone (MCH)