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The fact that ordered parts of a protein molecule are insensitive to proteolysis gives raise to the sequence/structure paradigm of limited proteolysis, where higher order structure and not primary sequence is the main determinant of the site of initial hydrolysis (Hubbard, 1998)

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Experimental Protocol In deeply anesthetized rats (intraperitoneal (ip) injected 40 mg/kg thiopental (Rotexmedica, Germany) and 10 mg/kg diazepam (Apaurin

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