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Proposed future directions include advanced nanodelivery systems, eutectic technologies, and precision nutrition strategies, which together could accelerate the translation of these natural compounds from the laboratory to clinical application

GSH/GSSG-Glo assay GSH/GSSG ratio of CuCl 2 -treated HepG2 and HepG2 ATP7B-KO cells was measured by GSH/GSSG-Glo Assay (Promega) according to manufactures instructions

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Important Notes Sample concentrations should be estimated before being used in the assay and a proper dilution factor should be selected to make the diluted target protein concentration fall in the optimal detection range of this kit