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Live CD3 + T cells were sorted from the airway (CD45 + CD90.2 - cells in BALF), the airway-parenchyma interface (air-par, CD45 + CD90.2 - cells in lungs), the parenchyma (CD45 - CD90.2 - cells in lungs), and the spleen into cold buffer (BD Rhapsody Cartridge Reagent Kit, 633731, BD, Biosciences) using a BD FACSAria III cell sorter

Differences between the effects of conventional cigarettes, e-cigarettes and dual product use on urine cotinine levels

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For some, it can also amplify existing issues such as anxiety