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Induction of immune tolerance by activation of CD8+ T suppressor/regulatory cells in lupus-prone mice

RUO Disclaimer The BPC-157 + TB-500 Blend (10mg/10mg) is an investigational research compound intended solely for in vitro laboratory research and in vivo animal studies

A 35 Ultra Semi diamond knife (Diatome) mounted on a UC7 ultramicrotome (Leica) was used to cut alternating series of sections onto formvar-coated copper mesh grids at two different thicknesses: (i) 60 nm-thick sections were visually inspected to assess ultrastructural preservation and synapse density under an 80kV LEO 912 transmission electron microscope (Zeiss), and (ii) 250 nm-thick sections were coated on both surfaces with Protein A-conjugated 10 nm gold fiducial particles (Cell Microscopy Core Products, University Medical Center Utrecht, The Netherlands) in preparation for transmission electron tomography

These results did not differ in patients with early (before-) or late (after hospital discharge) recanalization