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(Nature, 2014) used an antisense oligonucleotide to knock down NNMT in mice and reported reduced fat-mass gain, better glucose tolerance, and lower liver triglycerides the founding paper for the whole NNMT-inhibitor field

Importantly, this proof-of-concept study showed that the relative abundance of Lactobacillus , a genus associated with weight loss 58 , was uniquely increased and Erysipelatoclostridium was distinctly decreased in mice given 5A-1MQ treatment and an LD switch compared to mice switched to LD and given vehicle treatment

Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a sharps container afterward