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In brief, HEK293T cells (American Type Culture Collection (ATCC), CRL-3216) were cultured in complete DMEM (Gibco, 11965-092) supplemented with 10% (v/v) FBS (VWR, 97068-085) and 1% penicillinstreptomycin (VWR, 16777-164) at 37 C under 5% CO 2

FIGURE 1 As SmTGR can act as both a TrxR and GR, when comparing the secondary structure of SmTGR with mammalian TrxR and GR one can infer which portion of the enzyme is likely responsible for which chemistry

Understanding these practical requirements is part of getting started with peptides effectively

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