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Bone marrow was flushed from femur and tibia of each mouse, using a 25 gauge needle with approximately 500 l of isolation buffer (PBS, 2% FBS and EDTA) per bone into 1 mL Eppendorf tubes

BRD4 recruits G9a (EHMT2) to ATG loci such as WIPI1 , GABARAPL1, LAMP1 , and CTSD , promoting H3K9 dimethylation and transcriptional repression