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In 1908, Freud said that he occasionally read Nietzsche and was strongly fascinated by his writings, but did not study him, because he found Nietzsche's "intuitive insights" resembled his own work at the time too much, and also because he was overwhelmed by the "wealth of ideas" he encountered when he read Nietzsche

After vortexing for 60 s, the mixture was incubated for 10 min at room temperature, and the samples were centrifuged at 12,000 rpm for 20 min at 4C