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Rahimnia SM, et al

Cells transformed with empty plasmids served as controls for the subtraction of fluorescence background

About 100 mg (dry weight) of immobilization carriers were collected and weighed in a 15 mL tube, N,N -dimethyl-4-aminopyridine (DMAP) (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) and N,N' -disuccinimidyl carbonate (DSC) (Tokyo Chemical Industry, Tokyo, Japan) were weighed to 1.51- and 3.16-times the weight of the immobilization carrier, respectively

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