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4 and the concentration of LMW copper is given by: where [L] is the concentration of the competing ligand (His), K 1 and K 2 correspond to the dissociation constants of CuHis and CuHis 2 complexes ( K 1 = 3.7 10 9 M, K 2 = 4.7 10 7 M, pH = 7.4 31 )

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