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Practical Implication: This research validates that topical GHK-Cu formulations achieve meaningful tissue concentrations, supporting the rationale for both cosmetic and research applications

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337,338,339 GLP-1 can exert its anti-inflammatory effects by activating its receptor, GLP-1R

Chelating this zinc signal with the membrane-permeable chelator TPEN effectively blocks the activation of LPS-induced signaling pathways, including the p38 MAPK, extracellular-signal regulated kinases 1/2 (ERK1/2) and NF-B pathways, ultimately inhibiting the release of proinflammatory cytokines such as TNF- [18]