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Reads were aligned to the ENSEMBL Homo_sapiens.GRCh38.dna.primary_assembly genome using the ENSEMBL Homo_sapiens.GRCh38.100 transcript [82] annotation file with STAR [83] splice-aware RNA-seq alignment tool in paired mode allowing maximum multimapping of 3

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[265] In July 2021, Carlson told Time magazine that the Republican Party is "inept and bad at governing" and "much more effective as an oppositional force than it is as a governing party"

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