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Plant Physiol 132:22482255 Alfafara CG, Kanda A, Shioi T, Shimizu H, Shioya S, Suga K (1992a) Effect of amino acids on glutathione production by Saccharomyces cerevisiae

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The cells were collected by centrifugation at 2000 g for 5 min and washed in phosphate buffered saline (8 gL 1 NaCl, 0.2 gL 1 KCl, 1.44 gL 1 Na 2 PO 4 , 0.24 g L 1 KH 2 PO 4 , at pH 7.5)