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Xiong Y, Yang Y, Yang J, Chai H, Li Y, Yang J, et al

Streptococcus , Enterococcus , and Clostridium further metabolize simple substrates into volatile fatty acids (VFAs), ethanol, and hydrogen, which methanogens ultimately convert to methane 38

Translational success will require (i) rigorous preclinical-to-clinical pipelines that include long-term toxicity and biodistribution studies, (ii) GMP- compliant manufacturing to ensure reproducibility and scalability, (iii) adaptive trial designs capable of evaluating patient-specific nanotherapies, and (iv) clearer regulatory alignment across FDA/EMA to classify and approve multifunctional nanoplatforms