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Engaging in regular physical activity, such as aerobic exercise and strength training, to enhance blood circulation and muscle tone

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These samples were frozen at 20 C until 2013 when total cotinine and total trans-3-hydroxy-cotinine (hereafter referred to as cotinine or 3OH-cotinine, respectively) were measured after enzymatic hydrolyses by two-dimensional liquid chromatography linked with tandem mass spectrometry and quantification via isotope dilution, as described before [12]