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Several reports demonstrated that ferroptosis is induced by loss of GPx4 activity due to the depletion of intracellular glutathione via the anti-cancer drug Erastin-mediated inhibition of the xCT cysteine transporter 37 , or by direct binding of RSL3 to selenocysteine in the GPx4 enzymatic active site 8

That process, called liquid chromatography, will separate the complex mixture of compounds into their individual chemical components

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