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The three formulas the calculator uses: Concentration (mg/mL) = vial mg bacteriostatic water mL Units to draw = (target mcg (vial mg 1000)) water mL 100 Doses per vial = (vial mg 1000) target mcg, rounded down A 5 mg vial reconstituted with 2 mL of bacteriostatic water is 2.5 mg/mL, or 25 mcg per unit