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Possible process of compromised immunotolerance in women is asymmetrical X chromosome inactivation (XCI), which cause evade from the presentation of X-linked self-antigens in the thymus and following deterioration of T cell tolerance

Cells resuspended in the growth medium (GM, DMEM containing 10% fetal bovine serum (FBS, Life Technologies) and 1% anti-anti), and the single cells were seeded in a cell culture flask

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