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With the rapid development of high-throughput sequencing technology, genomics has gradually transitioned from basic research to clinical applications, providing strong technical support for the precise diagnosis and targeted treatment of cancer (Mukherjee, 2019)

doi: 10.1210/jcem.87.6.8739 46

The suspension was incubated in a boiling water bath for 10 min, cooled to room temperature, and centrifuged at 25 C, 4,000 g for 10 min