In this step, RPMI culture medium (Bio-IDEA), which is suitable for PBMCs cell culture, along with FBS and trypan blue dye, was utilized to identification of living and dead cells
A play on the saying "what a mess"
The town was literally divided into hot and cold sides, depending on whether a family lived on land directly above the tunnels of fire or above tunnels the fire had not yet reached

Acknowledgements This study was funded by the Natural Science Foundation of Guangdong Province (2023A1515011728), Plan on enhancing scientific research in GMU (02-410-230221XM), Project of Guangzhou Municipal Science and Technology Bureau (2023A03J0835), the Guangzhou Municipal Health Commission (2023C-TS26), Opening Foundation of Jiangsu Key Laboratory of Neurodegeneration, Nanjing Medical University (KF202202), Open Project Program of State Key Laboratory of Virtual Reality Technology and Systems, Beihang University (VRLAB2022 B02), and Shanghai Key Laboratory of Psychotic Disorders Open Grant (21-K03), Guangzhou Key R&D Program Agriculture and Social Development Science and Technology Project (202206010034), Guangzhou Municipal Basic Research Project jointly funded by municipal schools (institutes) (2023A03J0834), Guangzhou High-level Clinical Key Specialty, and Guangzhou Research-oriented Hospital