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While sub-threshold concentrations of each ligand alone did not alter basal spinogenesis, combined sub-threshold concentrations of 30 " M Dihexa and 2.5 ng/ml HGF or 10 " 13 M Nlel -AnglV and 2.5 ng/ml of HGF produced a near ceiling effect, similar to biological responsive doses of each ligand alone (Figure 1 1 A and B)

Response patterns vary by longevity goal: sleep and circadian rhythm respond on different curves than recovery rhythm, post-stress aging, or cellular aging markers

Discard the supernatant, re-suspend the pellet in 3 ml of 5% (w/v) sulfosalicylic acid and mix by vortexing

Rev Clin Exp Hematol 7(2):160-90, 2003