Immunoprecipitation Technique size:For 6 groups of 4-5 or 24-30 students. Crude extracts of plasmid DNA

Crude extracts of plasmid DNA were prepared from cells grown in selective media and analyzed by agarose gel electrophoresis

Samples of induced and uninduced cells containing equivalent numbers of cells were centrifuged

Modified RNA containing pyrimidine-2´-fluoro-dNMPs

  The kit is supplied with variations of the main protocol to educate students on the different conditions required for purification from different tissues

Immunoprecipitation Technique size:For 6 groups of 4-5 or 24-30 students. Crude extracts of plasmid DNAImmunoprecipitation is a routinely used technique that removes a protein or peptide, which specifically reacts with an antibody, from a solution. The name of the technique is a misnomer as the interaction of the peptide or protein with the antibody does not cause precipitation. The precipitation is caused by an immunoglobulin binding protein, such as protein A or protein G, immobilized to a solid support or bead. The protein A or G binds the antibody