Cytidine 5'-diphosphate (CDP) sodium salt hydrate ECloni5alpha Transformations were performed using 50

Transformations were performed using 50 µl of competent cells and either supercoiled DNAs of the indicated sizes or a 1-µl aliquot from a standard 10-µl ligation reaction

ELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers

Contaminating Activity Assays: EpiScript RT is free of detectable exonuclease

The reagent contains disruptive and protective properties of guanidine isothiocyanate and β-mercaptoethanol to inactivate the ribonucleases present in cell extracts

Cytidine 5'-diphosphate (CDP) sodium salt hydrate ECloni5alpha Transformations were performed using 50