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Once calibration had finished, the cell plate was inserted into the analyser, and OCR was determined at basal and mitochondrial stress conditions by triplicate measurement cycles of 0.5 min mixing, 0.5 min waiting and 2 min measuring, carried out at baseline and after each port injection

Further investigation of the protein dynamics suggested that L585 contributes to the flexibility of DIII, which undergoes large conformational changes upon FcRn engagement 52,59,60

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