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Description

Cytokine production after LPS induction was measured with ELISA kits for IL-6 (R&D, M6000B-1) and TNF (BioLegend, 430907)

doi: 10.1038/s41419-021-04284-5

J Cell Neurosci Oxid Stress 11:861873

After centrifuging the cell suspension at 50g for 3 min, collect cell pellet and purify with 40% percoll (Cytiva, MA, USA) to increase the percentage of viable hepatocytes